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Buying a New qPCR Machine? Here’s What You Should Know First

Price is often one of the first and most important factors lab managers consider when purchasing a new qPCR machine. One reason for this is that it obviously has a direct and immediate impact on your lab’s budget.

However, there are also a few more hidden costs, such as the running cost of the cycler, that are far less obvious as they are spread over the years. And they can make a big difference. Let’s explore those more in detail.

qPCR plate on PlatR

Understanding qPCR Efficiency and Why It Can Exceed 100%

Quantitative polymerase chain reaction (or qPCR) is a well-established assay for nucleic acid quantification and is still regarded as the method of choice in most areas of molecular biology. Though different types of qPCR quantification exist (absolute and relative), determining the amplification efficiency should be among the first things to do when setting up a qPCR assay. Understanding efficiency and how to calculate it is crucial for accurate data interpretation.

qPCR technology

Real-Time PCR (qPCR) Technology Basics

The method of choice for nucleic acid (DNA, RNA) quantification in all areas of molecular biology is real-time PCR or quantitative PCR (qPCR). The method´s name derives from the fact that the amplification of DNA by polymerase chain reaction (PCR) is monitored in real time. It is a quantitative method in contrast to conventional PCR, meaning that it enables the determination of exact amounts (relative or absolute) of amplified DNA in samples.