Quantitative PCR (or qPCR), microarrays and RNA sequencing are all very valuable assays for in-depth gene expression analysis. But which one should you choose over the other for your next big experiment? Well quite frankly, it mostly depends on the goals of the project, your budget, and the organism of interest.
The method of choice for nucleic acid (DNA, RNA) quantification in all areas of molecular biology is real-time PCR or quantitative PCR (qPCR). The method´s name derives from the fact that the amplification of DNA by polymerase chain reaction (PCR) is monitored in real time. It is a quantitative method in contrast to conventional PCR, meaning that it enables the determination of exact amounts (relative or absolute) of amplified DNA in samples.