Price is often one of the first and most important factors lab managers consider when purchasing a new qPCR machine. One reason for this is that it obviously has a direct and immediate impact on your lab’s budget.
However, there are also a few more hidden costs, such as the running cost of the cycler, that are far less obvious as they are spread over the years. And they can make a big difference. Let’s explore those more in detail.
Quantitative polymerase chain reaction (or qPCR) is a well-established assay for nucleic acid quantification and is still regarded as the method of choice in most areas of molecular biology. Though different types of qPCR quantification exist (absolute and relative), determining the amplification efficiency should be among the first things to do when setting up a qPCR assay. Understanding efficiency and how to calculate it is crucial for accurate data interpretation.
For many of us the process of pipetting transitioned into a subconscious routine to which we devote little thought after only a little practice, despite quality assurance placing great emphasis on liquid handling, pipette accuracy, repair and maintenance. Nevertheless, ensuring the competence of pipette operators (lab employees) is a way-too-often often neglected activity.