Science and Software

Spring of ideas from experienced researchers and software experts

 

qPCR plate on PlatR

Understanding qPCR Efficiency and Why It Can Exceed 100%

Quantitative polymerase chain reaction (or qPCR) is a well-established assay for nucleic acid quantification and is still regarded as the method of choice in most areas of molecular biology. Though different types of qPCR quantification exist (absolute and relative), determining the amplification efficiency should be among the first things to do when setting up a qPCR assay. Understanding efficiency and how to calculate it is crucial for accurate data interpretation.

Pipetting with Pipetting Aid PlatR

12 Easy Tips to Improve Your Pipetting Technique

For many of us the process of pipetting transitioned into a subconscious routine to which we devote little thought after only a little practice, despite quality assurance placing great emphasis on liquid handling, pipette accuracy, repair and maintenance. Nevertheless, ensuring the competence of pipette operators (lab employees) is a way-too-often often neglected activity.

6 Reasons Why You Should Outsource Software Development

6 Reasons to Outsource Software Development in 2017 (With Tips)

Globalization is driving competitiveness in the business space at an unprecedented pace, pushing companies to constantly look for lean ways of improving their business. A clever place to start is resource allocation. When planning a new project, the first task is to evaluate its scope and allocate your talent and finances accordingly. This might sound straightforward, but it’s actually a treasure chest of overlooked opportunities for business optimization and company growth.

qPCR technology

Real-Time PCR (qPCR) Technology Basics

The method of choice for nucleic acid (DNA, RNA) quantification in all areas of molecular biology is real-time PCR or quantitative PCR (qPCR). The method´s name derives from the fact that the amplification of DNA by polymerase chain reaction (PCR) is monitored in real time. It is a quantitative method in contrast to conventional PCR, meaning that it enables the determination of exact amounts (relative or absolute) of amplified DNA in samples.

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